Genetic analysis of zinc-finger nuclease-induced gene
نویسندگان
چکیده
Zinc-finger nucleases can be designed to cleave arbitrary genomic sequences, which stimulates localized mutagenesis and targeted gene replacement. Both outcomes depend on normal cellular processes of DNA repair, and this study examines the pathways responsible. Gene replacement by homologous recombination depends on the activity of the Rad51 protein. Much of local mutagenesis depends on DNA ligase IV, Lig4. In the absence of Rad51, repair is biased toward mutagenesis; in the absence of Lig4, a strong shift toward gene replacement is seen.
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